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Capillary liquid chromatographic fingerprint used for discrimination of Zingiber montanum from related species
Authors:Mohamad Rafi  Lee Wah Lim  Toyohide Takeuchi  Latifah Kosim Darusman
Institution:1. Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan
2. Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jalan Agatis Kampus IPB Dramaga, Bogor, 16680, Indonesia
3. Biopharmaca Research Center-Research and Community Empowerment Institute, Bogor Agricultural University, Jalan Taman Kencana No. 3 Kampus IPB Taman Kencana, Bogor, 16128, Indonesia
Abstract:Fingerprint analysis using capillary liquid chromatography (CLC) has been developed for discrimination of Zingiber montanum (ZM) from related species, for example Z. americans (ZA) and Z. zerumbet (ZZ). By comparing the fingerprint chromatograms of ZM, ZA, and ZZ we could identify ZM samples and discriminate them from ZA and ZZ by using their marker peaks. We also combined CLC fingerprint with multivariate analysis, including principal-component analysis (PCA) and canonical variate analysis (CVA); all three species were discriminated successfully. This result indicates that CLC fingerprint analysis in combination with PCA and CVA can be used for discrimination of ZM samples from samples of related species.
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