Evaluation of the enantiomeric selectivity in the chiral ligand-exchange chromatography of amino acids by a computational model |
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Authors: | Natalini Benedetto Marinozzi Maura Sardella Roccaldo Macchiarulo Antonio Pellicciari Roberto |
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Institution: | Dipartimento di Chimica e Tecnologia del Farmaco, Università degli Studi di Perugia, Via del Liceo 1, 06123 Perugia, Italy. natalini@iris.chimfarm.unipg.it |
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Abstract: | The chromatographic resolution of enantiomeric amino acids is accomplished on a reversed phase column using aqueous mobile phase containing the chiral reagent N,N-dimethyl-S-phenylalanine-Cu(II). The separation is a result of the whole interaction between the diastereomeric complex surface and the mixed stationary phase realized by the dynamic coating of the RP-18 carbon chains layer. The elution order seems to be related to the different water coordination capability on copper ion in the formation of the mixed ternary complexes. |
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Keywords: | Enantiomer separation Computational model Solvent accessible area Elution order Amino acids |
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