Improved and isotropic resolution in tomographic diffractive microscopy combining sample and illumination rotation |
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Authors: | Stanislas Vertu Jens Flügge Jean-Jacques Delaunay Olivier Haeberlé |
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Institution: | (1) Division of Bioengineering, National University of Singapore, 9 Engineering Drive, Singapore, 117576, Singapore;(2) Singapore Eye Research Institute, 11, Third Hospital Avenue, #05-00, Singapore, 168751, Singapore;(3) Computation and Systems Biology, Singapore-MIT Alliance, National University of Singapore, Singapore, 117576, Singapore;(4) Department of Diagnostic Radiology, National University of Singapore, 5 Lower Kent Ridge Road, Singapore, 119074, Singapore |
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Abstract: | Tomographic Diffractive Microscopy is a technique, which permits to image transparent living specimens in three dimensions
without staining. It is commonly implemented in two configurations, by either rotating the sample illumination keeping the
specimen fixed, or by rotating the sample using a fixed illumination. Under the first-order Born approximation, the volume
of the frequency domain that can be mapped with the rotating illumination method has the shape of a “doughnut”, which exhibits
a so-called “missing cone” of non-captured frequencies, responsible for the strong resolution anisotropy characteristic of
transmission microscopes. When rotating the sample, the resolution is almost isotropic, but the set of captured frequencies
still exhibits a missing part, the shape of which resembles that of an apple core. Furthermore, its maximal extension is reduced
compared to tomography with rotating illumination. We propose various configurations for tomographic diffractive microscopy,
which combine both approaches, and aim at obtaining a high and isotropic resolution. We illustrate with simulations the expected imaging performances of these configurations. |
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