Dielectrophoresis assisted immuno-capture and detection of foodborne pathogenic bacteria in biochips |
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Authors: | Liju Yang |
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Institution: | Biomanufacturing Research Institute and Technology Enterprise (BRITE), and Department of Pharmaceutical Sciences, North Carolina Central University, 1801 Fayetteville St, Durham, NC 27707, USA |
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Abstract: | This study integrated dielectrophoresis (DEP) with non-flow through biochips to enhance the immuno-capture and detection of foodborne pathogenic bacteria. It demonstrated two major functions provided by DEP to improve the chip performance: (i) concentrating bacterial cells from the suspension to different locations on the chip surface by positive and negative DEP; (ii) making the cells in close contact with the immobilized antibodies on the chip surface so that immuno-capture efficiency can be dramatically enhanced.The microchip achieved the immuno-capture efficiencies of ∼56.0% and ∼64.0% to Salmonella cells with 15 and 30 min DEP, respectively, which were considerably higher than those of ∼10.4% and ∼17.6% for 15 and 30 min immuno-capture without DEP. The immuno-captured bacterial cells were detected by the sandwich format ELISA on the chips. The final absorbance signals were enhanced by DEP assisted immuno-capture by 64.7-105.2% for the samples containing 103-106 cells/20 μl. The integration of DEP with the biochips has the potential to advance the chip-based immunoassay methods for microbial detection. |
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Keywords: | Bacteria detection Dielectrophoresis Immuno-capture Immunoassay Biochip |
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