RNA PROTEIN CROSSLINKS INTRODUCED INTO E. coli RIBOSOMES BY USE OF THE INTRINSIC PROBE 4-THIOURIDINE |
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| Authors: | Eliane Hajnsdorf Yolande Lemaigre Dubreuil Roberto Bezerra Alain Favre Alain Expert-BezanÇon |
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| Institution: | Groupe de Photobiologie Moléculaire, Institut Jacques Monod, CNRS, UniversitéParis VII 2 Place Jussieu 75251 Paris Cedex 05, France |
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| Abstract: | Abstract— 70S Ribosome substituted by the uridine photoactivable analogue 4-thiouridine has been prepared by an in vivo method (substitution level 4.5%). The r-proteins crosslinked to 16S and 23S rRNA before and after 366-nm photoactivation were identified. Proteins S2-S7-S9/11-S18 are found linked to 16S RNA in dark-prepared 30S subunits. Illumination increases uniformly their binding by a factor of 2.5. Similarly, proteins L5-L15-L18-L23-L28-L32 are found crosslinked to 23S RNA in dark-prepared 50S subunits. Photoactivation increases their binding but in addition promotes the covalent linking of proteins L1-L3-L4. |
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