Improving surface plasmon resonance imaging of DNA by creating new gold and silver based surface nanostructures |
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Authors: | Stefano Mariani Maria Laura Ermini Simona Scarano Francesca Bellissima Massimo Bonini Debora Berti Maria Minunni |
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Institution: | 1. Department of Chemistry “Ugo Schiff” and CSGI, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, FI, Italy
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Abstract: | The use of nanoparticles (NPs) can substantially improve the analytical performance of surface plasmon resonance imaging (SPRi) in general, and in DNA sensing in particular. In this work, we report on the modification of the gold surface of commercial biochips with gold nanospheres, silica-coated gold nanoshells, and silver nanoprisms, respectively. The NPs were tethered onto the surface of the chip and functionalized with a DNA probe. The effects of tethering conditions and varying nanostructures on the SPRi signals were evaluated via hybridization assays. The results showed that coupling between planar surface plasmons and electric fields, generated by localized surface plasmons of the NPs, is mandatory for signal enhancement. Silver nanoprisms gave the best results in improving the signal change at a target DNA concentration of <50 nM by +50 % (compared to a conventional SPRi chip). The limit of detection for the target DNA was 0.5 nM which is 5 times less than in conventional SPRi. Figure ? |
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