Spectrophotometric determination of ascorbic acid with potassium hexacyanoferrate(III) |
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| Authors: | Burger N Karas-Gasparec V |
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| Institution: | Department of Chemistry and Biochemistry, Faculty of Medicine, University of Zagreb 41000 Zagreb, Croatia, Salata 3, Yugoslavia. |
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| Abstract: | A new, simple and rapid method of determination of ascorbic acid in amounts of 45-360 mug is described. The ascorbic acid is determined spectrophotometrically at 420 nm from the decrease in absorbance it causes in 1 x 10(-3)M hexacyanoferrate(III) in McIlvaine buffer at pH 5.2. The proposed method is suitable for the determination of ascorbic acid in pharmaceutical preparations and probably natural products. |
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