| 红车轴草总异黄酮成分DNA结合剂的超滤质谱筛选 |
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| 引用本文： | 马蕾,王兆伏,陈丽娜,宋凤瑞,刘志强,刘淑莹.红车轴草总异黄酮成分DNA结合剂的超滤质谱筛选[J].高等学校化学学报,2013,34(2):331-335. |
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| 作者姓名： | 马蕾 王兆伏 陈丽娜 宋凤瑞 刘志强 刘淑莹 |
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| 作者单位： | 1. 中国科学院长春应用化学研究所, 长春质谱中心, 长春 130022;
2. 中国科学院大学, 北京 100049 |
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| 基金项目： | 国家自然科学基金(批准号:21073178,21175128)资助 |
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| 摘 要： | 采用离心超滤和液相色谱-质谱联用的方法, 从红车轴草异黄酮提取物中筛选DNA结合剂. 结果表明, 红车轴草中10种异黄酮成分与DNA具有不同的结合能力. 采用液相色谱-串联质谱联用技术对其结构进行了鉴定, DNA结合能力较强的5种化合物分别是德鸢尾素-4'-O-β-D-葡萄糖苷、 德鸢尾素、 黄豆黄葡萄糖苷、 红车轴草素和鹰嘴豆牙素A. 为从中药提取物等复杂体系筛选并鉴定DNA结合剂建立了快速的超滤质谱平台.
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| 关 键 词： | 红车轴草 DNA 离心超滤 液质联用 |
| 收稿时间： | 2012-05-14 |
Screening DNA Binders from Isoflavone Extracts of Red Clover by Centrifugal Ultrafiltration-LC-MS~n |
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| MA Lei,WANG Zhao-Fu,CHEN Li-Na,SONG Feng-Rui,LIU Zhi-Qiang,LIU Shu-Ying.Screening DNA Binders from Isoflavone Extracts of Red Clover by Centrifugal Ultrafiltration-LC-MS~n[J].Chemical Research In Chinese Universities,2013,34(2):331-335. |
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| Authors: | MA Lei WANG Zhao-Fu CHEN Li-Na SONG Feng-Rui LIU Zhi-Qiang LIU Shu-Ying |
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| Institution: | 1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Centrifugal ultrafiltration and LC-MS methods were combined to screen DNA-binding agents from traditional Chinese medicine red clover isoflavone extracts. The results show that 10 red clover isoflavones have different binding degrees with DNA. The sample was analyzed by LC-MSn for identifying structures. Among these flavones studied, the strongest five potential DNA-binding agents are irilone-4'-O-β-D-glucoside, glycitin, pratensein, irilone and biochanin A, respectively. A rapid centrifugal ultrafiltration-LC-MS platform for screening and identification of DNA binders from the herbal extract and other complex system was established. |
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| Keywords: | Trifolium pratense L Trifolium pratense L" target="_blank">')" href="#">Trifolium pratense L DNA Centrifugal ultrafiltration LC-MS |
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