A specific probe for two-photon fluorescence lysosomal imaging |
| |
Authors: | Zhiguo Wu Mingliang Tang Tian Tian Jianguo Wu Yuanlin Deng Xiaohu Dong Zheng Tan Xiaocheng Weng Zhihong Liu Chunjiang Wang Xiang Zhou |
| |
Institution: | 1. State Key Laboratory of Virology, College of Chemistry and Molecular Sciences, Wuhan University, Hubei, Wuhan, PR China;2. State Key Laboratory of Natural and Biomimetic Drugs, Perking University, Hubei, Wuhan 430072, PR China;3. Institute of Zoology, Chinese Academy Science, PR China |
| |
Abstract: | Lysosomes are vital organelles in physiological processes, as they receive and degrade macromolecules from the secretory and endocytic procedures. Evidences have shown that lysosomes were related to oncogenic activation and cancer progression, so lysosomes targeting and imaging probes make them convenient to be observed. In this study, a lysosome specific probe W-7 was designed and synthesized via convenient one-pot reaction and Heck reaction. This probe was derived from Tröger's base with a dimethylaminomethyl end group. The optical properties of this compound were measured. W-7 also showed two-photon absorption (TPA) effect by using laser excitation at the wavelength of infrared light. In vivo experiment, W-7 showed high specificity and selectivity for lysosomes in living cells (HeLa cells, MRC-5 cells and NRK cells), compared with LT Red, GT Red and MT Red (R = 0.96). Two-photon fluorescence images of HeLa cells stained by W-7 were obtained. And high resolution 3D reconstruction of lysosomes in one HeLa cell was provided by using two-photon confocal microscopy. The anantioseparation of racemic W-7 was carried out by chiral-HPLC, and the two enantiomers showed no significant difference in lysosomes imaging. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|