Development and validation of a high performance liquid chromatographic method for the simultaneous determination of potassium clavulanate and cefadroxil in synthetically prepared tablets |
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Authors: | S Sharif I U Khan M Ashfaq M S Iqbal S Ahmad |
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Institution: | 1.Department of Chemistry, Faculty of Science and Technology,Government College University,Lahore,Pakistan;2.Department of Chemistry,University of Gujrat,Gujrat,Pakistan;3.Pharmagen Ltd.,Lahore,Pakistan |
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Abstract: | A simple, sensitive and rapid high performance liquid chromatographic method was developed and validated for the simultaneous
determination of potassium clavulanate and cefadroxil in synthetically prepared tablets. Chromatographic separation and detection
was carried out on a C-18 column using 0.05 M potassium dihydrogen phosphate buffer (pH 5.0) and acetonitrile in the ratio
of 94: 06 (v/v) as mobile phase at wavelength of 225 nm. The method was linear in the concentration range of 3.75–22.5 μg/mL
for potassium clavulanate and 15–90 μg/mL for cefadroxil. The flow rate was 1.0 mL/min and the total analysis time was less
than 10 min. The mean recoveries was found to be greater than 99% with RSD less than 1.0%. The proposed method was validated
by performing linearity, recovery, specificity, robustness, LOD/LOQ and within-day and between-day precision. The chromatographic
results obtained from the synthetically prepared tablets show that the method is highly precise and accurate for the simultaneous
quantitation of clavulanate potassium and cefadroxil. |
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