白眉蝮蛇蛇毒及蛇毒酶的激光解吸飞行时间质谱研究

Perkins等1]用MALDI/TOF/MS对不同种类蛇毒中神经毒素进行了分析,彭嘉柔等2,3]对江浙蝮蛇和蛇毒粗组份进行了初步质谱表征.但蛇毒蛋白纯化困难,因此对单一组份的研究较少且不系统.本文以白眉蝮蛇蛇毒(AgkistrodonblomhoffiiUssurensis,ABUV)为原料,纯化得到了精氨酸酯酶(Arginineesterase,AEase)、磷脂酶A2(PhospholipaseA2,PLA2)、纤溶酶(fibrinolyticenzyme)和L-氨基酸氧化酶(L-aminoacidoxidase),并且用MALDI/TOF/MS法对它们和蛇毒粗毒进行了系统研究.1　实验部分1.1　仪器和药品　激光解吸质谱仪为美国Molecular公司L…

Mass Spectrometry Study on Snake Venoms and Enzymes

In this paper, three kinds of snake venoms and four kinds of enzymes (phospholipase A 2, fibrinolytic enzyme, arginine esterase and L amino acid oxidase) isolated from the snake venom were analyzed. As the snake venom was different, the MALDI/TOF/MS showed difference. The MALDI/TOF/MS determination results could be affected by the concentrations of snake venom enzymes. And the mechanisms of desorption and ionization was also given in this study. By using MALDI/TOF/MS we obtained the accurate molecular weights and homogeneities of the enzymes. The apparent characteristics of the positive MALDI/TOF/MS of enzymes composed by two subunits were also given out. The results showed that MALDI/TOF/MS is an effective analytic method for discovering new components from snake venom complexes. And it is reliable to use this method to determine the molecular weights and purifies of protein molecules. WT5HZ]