首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Identification of compounds from high‐fat and extra virgin olive oil‐supplemented diets in whole mouse liver extracts and isolated mitochondria using mass spectrometry
Authors:Gustavo Aparecido dos Santos  Mônica Siqueira Ferreira  Diogo Noin de Oliveira  Vanessa de Oliveira  Edilene S Siqueira‐Santos  Dennys Esper Corrêa Cintra  Roger Frigério Castilho  Lício Augusto Velloso  Rodrigo Ramos Catharino
Institution:1. INNOVARE Biomarkers Laboratory, School of Medical Sciences, University of Campinas, Campinas, SP, Brazil;2. Sport Sciences Course, Faculty of Applied Sciences, University of Campinas, Campinas, SP, Brazil;3. Experimental Neurodegeneration Laboratory, School of Medical Sciences, University of Campinas, Campinas, SP, Brazil;4. Laboratory of Cell Signaling, School of Medical Sciences, University of Campinas, Campinas, SP, Brazil
Abstract:Nonalcoholic steatohepatitis (NASH) is a fatty liver disorder that could be improved with extra virgin olive oil (EVOO) supplementation in diet. We propose the monitoring, in whole mouse liver extracts and in isolated mitochondria, of the absorption of compounds from three different diets: standard (CT), high‐fat (HFD) and high‐fat supplemented with EVOO (HFSO). Male mice were submitted to one of the following three diets: CT or HFD for 16 weeks or HFD for 8 weeks followed by additional 8 weeks with HFSO. Following this period, liver was extracted for histological evaluation, mitochondria isolation and mass spectrometry analyses. Diets, liver extracts and Percoll‐purified mitochondria were analyzed using ESI‐MS and the lipidomics approach. Morphological, histological and spectrometric results indicated a decrease in NASH severity with EVOO supplementation in comparison with animals maintained with HFD. Spectrometric data also demonstrated that some compounds presented on the diets are absorbed by the mitochondria. EVOO was shown to be a potential therapeutic alternative in food for NASH. Our results are in accordance with the proposition that the major factor that influences different responses to diets is their composition – and not only calories – especially when it comes to studies on obesity. Copyright © 2015 John Wiley & Sons, Ltd.
Keywords:diet  electrospray ionization mass spectrometry  extra virgin olive oil  nonalcoholic steatohepatitis  Omics
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号