| 以锌试剂显色法测定蛋白质的研究 |
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| 引用本文： | 迟燕华,李娜,庄稼,李克安,童沈阳.以锌试剂显色法测定蛋白质的研究[J].高等学校化学学报,1998,19(6):879-881. |
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| 作者姓名： | 迟燕华 李娜 庄稼 李克安 童沈阳 |
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| 作者单位： | 北京大学化学与分子工程学院, 北京, 100871 |
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| 摘 要： | 有关蛋白质染色测定的分光光度研究已有不少报道，其中有些已用于蛋白质的分析测定［１～４］．锌试剂常用于金属离子的分光光度法测定，但作为生物大分子分析试剂的研究，目前还未见报道．实验表明，在ｐＨ４左右的缓冲溶液中，锌试剂与ＢＳＡ反应生成有色复合物，吸收光...
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| 关 键 词： | 锌试剂 蛋白质 分光光度法 |
| 收稿时间： | 1997-07-24 |
The Spectrophotometric Determination of Proteins Using Zincon |
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| CHI Yan-Hua,LI Na,ZHUANG Jia,LI Ke-An,TONG Sheng-Yang.The Spectrophotometric Determination of Proteins Using Zincon[J].Chemical Research In Chinese Universities,1998,19(6):879-881. |
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| Authors: | CHI Yan-Hua LI Na ZHUANG Jia LI Ke-An TONG Sheng-Yang |
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| Institution: | Department of Chemistry, Peking University, Beijing, 100871 |
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| Abstract: | When BSA was added into zincon solution at pH 4.1, the purple colour was observed, which indicates the formation of zincon-BSA complex. The maximum absorption of complex was 564 nm and 594 nm with the apparent molar absorptivity of 1.5×106 L·mol-1 · cm-1 . The linear range of standard curve for BSAis 0～10 μg/mL, and the detection limit is 0.5 μg/mL. The basic reaction conditions were investigated. This proposed method was simple, sensitive, and reproducible. The analytical results of human serum albumin using this method is very satisfactory . |
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| Keywords: | Protein Zincon Spectrophotometry |
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