Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems |
| |
Authors: | Mojović Milos Spasojević Ivan Bacić Goran |
| |
Institution: | Faculty of Physical Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade. |
| |
Abstract: | We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (*H) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Baci?, G.; Mojovi?, M. Ann. N. Y. Acad. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (*H) adduct i.e., DEPMPO/H. An electrochemical system known to produce only *H radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|