蔗糖酯的酶催化区域选择性合成

酶可以区域选择性地合成蔗糖酯，枯草杆菌等蛋白酶被用来区域选择性合成 1'-O-蔗糖酯，且通常不接受长链（大于C_(12))脂肪酸作为酰基授体，而脂肪酶可 催化宽范围的脂肪酸，除了脂肪酶和蛋白酶外，研究发现抗体酶也能区域和立体选 择地酰化蔗糖。蔗糖二酯、线型蔗糖酯都可以通过酶催化区域选择性地合成。酶催 化合成蔗糖酯通常在二甲基甲酰胺、二甲基亚砜和吡啶等有毒溶剂中进行。近几年 来，无溶剂条件下酶催化合成蔗糖酯，以及用叔丁醇等毒性较小的溶剂代替毒性较 大的溶剂的研究，也越来越受重视。

Enzyme-Catalyzed Regioselective Synthesis of Surcrose Esters

Enzymes have been used to catalyze the reaction for the synthesis of sucrose esters. Subtilisin and other proteases are used for the synthesis of l'-O-acyl sucrose, but do not normally accept long-chain carboxylic acids as acyl donors. However, lipases perform well with a wide range of fatty acid. In addition, it was discovered that abzymes can be used for the regioselective acylation of sucrose. Diesters and linear polyester have been obtained by the enzymatic synthesis. Enzymatic regioselective synthesis of sucrose esters is commonly carried out in dimethylformamide, dimethyl sulfoxide , pyridine and other solvents which are poisonous. In recent years, the studies on enzyme-catalyzed regioselective synthesis under solvent-free conditions and on processes of the use of less harmful solvents such as tert-butanol have been paid more attention.