| 具有甲状腺素脱碘酶活性的抗体酶的制备及性质研究 |
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| 引用本文： | 孙立伟,刘立岩,赵大庆.具有甲状腺素脱碘酶活性的抗体酶的制备及性质研究[J].高等学校化学学报,2002,23(12):2217-2220. |
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| 作者姓名： | 孙立伟 刘立岩 赵大庆 |
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| 作者单位： | 中国科学院长春应用化学研究所稀土化学与物理开放实验室, 长春130022 |
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| 基金项目： | 国家自然科学基金重大项目 (批准号 :29890 2 80 )资助 |
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| 摘 要： | 首次以具有适当疏水性的甲状腺素衍生物O-methyl-T4为半抗原,用杂交瘤技术得到相应的抗体(6E8),又经过苯甲基磺酰氟和NaHSe的化学修饰引入了催化基团硒代半胱氨酸,制备出具有较高甲状腺素脱碘酶活力的抗体酶(Se-6E8).用SDS-PAGE电泳对Se-6E8的纯度进行了鉴定,同时研究了其解离常数、温度及pH等酶学性质.结果表明,Se-6E8活力为2010U/μmolprotein,纯度达到电泳纯,催化反应的最适温度为57℃,最适pH为82.
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| 关 键 词： | 硒 甲状腺素 脱碘酶 抗体酶 |
| 文章编号： | 0251-0790(2002)12-2217-04 |
| 收稿时间： | 2002-03-26 |
Preparation and Properties of Abzyme with Thyroxine Deiodinase |
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| SUN Li-Wei,LIU Li-Yan,ZHAO Da-Qing.Preparation and Properties of Abzyme with Thyroxine Deiodinase[J].Chemical Research In Chinese Universities,2002,23(12):2217-2220. |
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| Authors: | SUN Li-Wei LIU Li-Yan ZHAO Da-Qing |
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| Institution: | Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China |
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| Abstract: | The abzyme (Se-6E8) with a higher thyroxine deiodinase activity was prepared by modifying the serine residues of monoclonal antibody (6E8) with phenylmethanesulfonyl fluoride and sodium hydrogen selenide, and the 6E8 against O-methyl-T4, which is a kind of thyroxine derivatives and was taken as a hapten for the first time. Two bands were found corresponding to the 5.5 kD heavy chain and the 2.7 kD light chain respectively by SDS-PAGE. The characteristics of dissociation constants, pH, and temperature were also studied. The results show that the activity of Se-6E8 is 2010 U/μmol protein , and the proper temperature and pH of the catalytic reactions is 57 ℃ and 8.2 respectively. |
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| Keywords: | Selenium Thyroxine Deiodinase Abzyme |
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