高碘酸氧化肝素抑制P-选择素介导的A375细胞粘附

摘要通过选择性化学修饰制备出具有低抗凝血活性的高碘酸氧化肝素, 并系统地分析了其抗肿瘤细胞粘附的活性. 研究结果表明, 具有低抗凝血活性的高碘酸氧化肝素仍然可以显著地抑制P-选择素介导的人恶性黑素瘤A375细胞与血小板间的粘附.

Periodate-oxidized Heparin Inhibiting P-selectin-mediated A375 Cell Adhesion

Abstract Numerous experiments  show that heparin affects tumor metastasis in various ways, and one of the mechanisms by which heparin inhibits metastasis is by blocking the P-selectin-based interaction of platelets with tumor cells. However, heparin′s significant anticoagulant properties and the potential of bleeding complications may contraindicate its use as an anti\|metastasis compound.  RO-heparin with low anticoagulant activity was tested as the inhibitor of human malignant melanoma A375 cells adhering to surface-immobilized platelets expressing P-selectin. Then we demonstrate that RO-heparin can block P-selectin-mediated A375 human melanoma cell adhesion, suggesting that it may has a potential value as a anti-metastatic, therapeutic agent.