毛细管电泳-原子光(质)谱联用技术在金属形态与生物分子相互作用研究中的应用

金属形态与生物分子相互作用研究对于揭示金属元素在正常生命过程、重大疾病的发生、诊断和治疗过程中的作用机理具有重要意义，发展研究金属元素形态与生物活性分子相互作用的新技术和新方法非常重要。简要总结了十余年来，在发展毛细管电泳与电热原子吸收光谱和毛细管电泳与电感耦合等离子体质谱在线联用新技术，及其在不同形态金属与生物分子相互作用机理研究方面的工作。这些工作利用毛细管电泳-原子光(质)谱联用技术不仅能够直接证明镉、锌、不同形态的汞、不同形态的锑与谷胱甘肽、DNA、人血清白蛋白、牛血清白蛋白间的相互作用，以及这些金属及其不同形态与生物分子加合物的生成，而且还能够测定相互作用的热力学参数、反应级数和动力学参数。另外，结合圆二色光谱、红外光谱光谱、拉曼光谱和X射线光电子能谱等实验手段，进一步研究了金属及其不同形态与生物分子作用对生物分子二级结构的影响及在生物分子上可能的结合位点等。

Novel Hyphenated Techniques of Atomic Spectrometry for Metal Species Interaction with Biomolecules

Trace metals may be adopted by biological systems to assist in the syntheses and metabolic functions of genes (DNA and RNA) and proteins in the environment. These metals may be beneficial or may pose a risk to humans and other life forms. Novel hybrid techniques are required for studies on the interaction between different metal species and biomolecules, which is significant for biology, biochemistry, nutrition, agriculture, medicine, pharmacy, and environmental science. In recent years, our group dwells on new hyphenated techniques based on capillary electrophoresis (CE), electrothermal atomic absorption spectrometry (ETAAS), and inductively coupled plasma mass spectroscopy (ICP-MS), and their application for different metal species interaction with biomolecules such as DNA, HSA, and GSH. The CE-ETAAS assay and CE-ICP-MS assay allow sensitively probing the level of biomolecules such as DNA damage by different metal species and extracting the kinetic and thermodynamic information on the interactions of different metal species with biomolecules, provides direct evidences for the formation of differentmetal species - biomolecule adducts. In addition, the consequent structural information were extracted from circular dichroism (CD) and X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, and Fourier transform infrared (FTIR) spectroscopy. The present works represent the most complete and extensive study to date on the interactions between different metal species with biomolecules, and also provide new evidences for and insights into the interactions of different metal species with biomolecules for further understanding of the toxicological effects of metal species.