Enzymatic electrodes nanostructured with functionalized carbon nanotubes for biofuel cell applications |
| |
Authors: | E Nazaruk K Sadowska J F Biernat J Rogalski G Ginalska R Bilewicz |
| |
Institution: | (1) Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland;(2) Chemical Faculty, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland;(3) Department of Biochemistry, Maria Curie Sklodowska University, Akademicka 19 Str, 20-031 Lublin, Poland;(4) Department of Biochemistry, Medical University of Lublin, Chodzki Street 1, 20-093 Lublin, Poland; |
| |
Abstract: | Nanostructured bioelectrodes were designed and assembled into a biofuel cell with no separating membrane. The glassy carbon
electrodes were modified with mediator-functionalized carbon nanotubes. Ferrocene (Fc) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate)
diammonium salt (ABTS) bound chemically to the carbon nanotubes were found useful as mediators of the enzyme catalyzed electrode
processes. Glucose oxidase from Aspergillus niger AM-11 and laccase from Cerrena unicolor C-139 were incorporated in a liquid-crystalline matrix-monoolein cubic phase. The carbon nanotubes–nanostructured electrode
surface was covered with the cubic phase film containing the enzyme and acted as the catalytic surface for the oxidation of
glucose and reduction of oxygen. Thanks to the mediating role of derivatized nanotubes the catalysis was almost ten times
more efficient than on the GCE electrodes: catalytic current of glucose oxidation was 1 mA cm−2 and oxygen reduction current exceeded 0.6 mA cm−2. The open circuit voltage of the biofuel cell was 0.43 V. Application of carbon nanotubes increased the maximum power output
of the constructed biofuel cell to 100 μW cm−2 without stirring of the solution which was ca. 100 times more efficient than using the same bioelectrodes without nanotubes
on the electrode surface. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|