| Imaging theory of nonlinear second harmonic and third harmonic generations in confocal microscopy |
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| 作者姓名： | TANG Zhilie XING Da & LIU Songhao . |
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| 基金项目： | 广东省自然科学基金，国家重点基础研究发展计划(973计划) |
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| 摘 要： | ItisreportedrecentlythatnonlinearopticalphenomenonofSHGandTHGhasbeenobservedinmanybiologicaltissues16].SHGandTHGhavebeenusedtoperformthethree-dimensionalimaginginlivingtissuesandhaveattractedmuchattentionrecently.TherearemanyadvantagesofusingSHGandTHGtoperformthethree-dimensionalimaginginlivingtissues,suchasnoninvasiveandnophotobleaching,inadditiontotheimagingpropertiesofmulti-photonfluorescenceimaging7—9].Firstly,unlikeinthesingle-andmulti-photonfluorescenceprocesses,onlyvirtualstat…
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| 收稿时间： | 22 January 2009 |
Imaging theory of nonlinear second harmonic and third harmonic generations in confocal microscopy |
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| TANG Zhilie,XING Da & LIU Songhao ..Imaging theory of nonlinear second harmonic and third harmonic generations in confocal microscopy[J].Science in China(Physics Astronomy),2004,47(1):8-16. |
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| Authors: | Email author" target="_blank">Zhilie?TangEmail author Da?Xing Songhao?Liu |
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| Institution: | 1. Department of Physics, South China Normal University, Guangzhou 510631, China
2. School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, China |
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| Abstract: | The imaging theory of nonlinear second harmonic generation (SHG) and third harmonic generation (THG) in confocal microscopy is presented in this paper. The nonlinear effect of SHG and THG on the imaging properties of confocal microscopy has been analyzed in detail by the imaging theory. It is proved that the imaging process of SHG and THG in confocal microscopy, which is different from conventional coherent imaging or incoherent imaging, can be divided into two different processes of coherent imaging. The three-dimensional point spread functions (3D-PSF) of SHG and THG confocal microscopy are derived based on the nonlinear principles of SHG and THG. The imaging properties of SHG and THG confocal microscopy are discussed in detail according to its 3D-PSF. It is shown that the resolution of SHG and THG confocal microscopy is higher than that of single- and two-photon confocal microscopy. |
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| Keywords: | second harmonic generation third harmonic generation confocal microscopy three-dimensional point spread function |
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