Superbeads: immobilization in "sweet" chemistry |
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Authors: | Nahalka Jozef Liu Ziye Chen Xi Wang Peng George |
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Institution: | Institute of Chemistry, Slovak Academy of Sciences Dúbravská cesta 9 SK-832 32 Bratislava, Slovak Republic. |
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Abstract: | Enzymatic oligosaccharide synthesis using recombinant glycosyltransferases is able to overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide regeneration cycles are necessary for the glycosylation. The multistep enzyme reaction can be efficiently carried out on superbeads that are prepared by immobilizing multienzyme mixtures on bead support through fused binding domains. |
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Keywords: | enzyme catalysis immobilization oligosaccharides sugar nucleotide regeneration |
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