| 食用菌中多种氨基甲酸酯类农药残留的超高效液相色谱-串联质谱法测定研究 |
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| 引用本文： | 赵颖,蒋施,金雁,姚家彪,刘瑜,徐宜宏,钟钰,吕红英,李梅.食用菌中多种氨基甲酸酯类农药残留的超高效液相色谱-串联质谱法测定研究[J].化学通报,2012(2):150-154. |
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| 作者姓名： | 赵颖 蒋施 金雁 姚家彪 刘瑜 徐宜宏 钟钰 吕红英 李梅 |
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| 作者单位： | 沈阳出入境检验检疫局综合技术中心 |
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| 基金项目： | “十一·五国家科技支撑计划“课题”农药与兽药残留确证检测技术研究”项目(2006BAK02A08-005)资助 |
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| 摘 要： | 建立了同时测定食用菌中19种氨基甲酸酯类农药(NMCs)残留的超高效液相色谱-串联质谱法(UPLC-MS/MS)。通过丙酮和乙腈(85∶15,V/V)提取,GPC法和PSA固相萃取柱两种方法净化,UPLC-MS/MS同时测定蘑菇、香菇、木耳和牛肝菌中19种氨基甲酸酯类农药,同位素稀释法定量。以GPC法净化,回收率为74.0%～93.2%;以PSA固相萃取柱净化,回收率为70.2%～91.8%,两种方法的检出限均为0.01mg/kg。各种NMCs在确定的添加范围内线性关系良好,线性相关系数R均大于0.99。所建立的检测方法实用、准确、灵敏度高。
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| 关 键 词： | 食用菌 氨基甲酸酯 超高效液相色谱-串联质谱 GPC PSA |
Study on UPLC-MS/MS Method for Determination of Carbamate Pesticide Residue in Mushrooms |
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| Zhao Ying,Jiang Shi,Jin Yan,Yao Jiabiao,Liu Yu, Xu Yihong,Zhong Yu,Lv Hongying,Li Mei.Study on UPLC-MS/MS Method for Determination of Carbamate Pesticide Residue in Mushrooms[J].Chemistry,2012(2):150-154. |
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| Authors: | Zhao Ying Jiang Shi Jin Yan Yao Jiabiao Liu Yu Xu Yihong Zhong Yu Lv Hongying Li Mei |
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| Institution: | (Technology Center,Shenyang Entry-Exist Inspection and Quarantine Bureau,Shenyang 110016) |
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| Abstract: | Methods to simultaneously determine 19 carbamate pesticides residues in mushrooms by liquid chromatography tandem mass spectrometry(UPLC-MS/MS)were established.The mushroom samples were extracted with acetone/acetonitrile(85∶15,V/V),purified by gel permeation chromatography(GPC) or solid phase extraction(SPE) and detected by UPLC-MS/MS with isotope internal standard method.Results showed that the recoveries ranged from 74.0% to 93.2% with the cleaning method of GPC and 70.2% to 91.8% with the cleaning method of PSA solid phase extraction.The limits of detection(LOD) was 0.01mg/kg.The method showed a good linear relationship with the correlation coefficients over 0.99.The established method is applied,accurate and high sensitive. |
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| Keywords: | Mushrooms Carbamate UPLC-MS/MS GPC PSA |
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