Hadamard Homonuclear Broadband Decoupled TOCSY NMR: Improved Efficacy in Detecting Long‐range Chemical Shift Correlations
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Authors: | Dr Veera Mohana Rao Kakita Prof Ramakrishna V Hosur |
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Institution: | 1. UM-DAE-Centre for Excellence in Basic Sciences, University of Mumbai, Mumbai, India;2. Department of Chemical Sciences, Tata Institute of Fundamental Research (TIFR), Mumbai, India |
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Abstract: | The importance of Hadamard encoding pulses in one‐dimensional pure shift yielded by the chirp excitation version of selective total correlation spectroscopy (1D PSYCHE–TOCSY) experiments is discussed for chemical‐shift analysis of complex natural products at ultrahigh resolution. Herein, we adapted Hn Hadamard matrices to 1D PSYCHE–TOCSY and observed an overall circa square root of n‐fold enhancement in the signal‐to‐noise (S/N) ratio when compared to conventional 1D PSYCHE–TOCSY recorded by refocusing only one spin at a time. This enhancement in S/N facilitates the observation of very weak long‐range chemical‐shift correlations from Hadamard‐encoded PSYCHE–TOCSY (HE–PSYCHE–TOCSY). The proposed method will have a significant impact on structure determination of complex isolated/ synthetic natural products. |
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Keywords: | chemical shift homodecoupling natural products nuclear magnetic resonance total correlation spectroscopy |
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