高效液相色谱法测定金葱粉中的罗丹明B含量

建立了金葱粉中罗丹明B的提取、富集净化和测定的方法.样品经加热萃取、聚酰胺吸附罗丹明B、碱液解吸、蒸发、定容,用高效液相色谱-紫外检测器进行测定.测定条件:色谱柱为ODS C18；流动相为甲醇-水(体积比为70∶30)；流速为1.5 mL/min；检测波长为550 nm.罗丹明B在0.14～22 μg/mL浓度范围内与...

DETERMINATION OF RHODAMINE B IN GLITTER POWDER BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

A method for the extraction, purification, enrichment and determination of the rhodamine B in glitter powder was established. The sample was extracted by heating, rhodamine B in it was adsorbed by polyamide, resolved by lye, evaporated, diluted to volume and determined by high performance liquid chromatography-UV detector. Measurement conditions： column was ODS C18, mobile phase was methanol-water（volume ratio was 70 ： 30）, flow rate was 1.5 mL/min, detection wavelength was 550 nm. Rhodamine B had good linear relationship with the peak area in the range of 0.14-22 μg/mL（t=0.999 8）. Recovery was 91.7%-104.7%. The RSD was 0,46%,1.45（n=5）%. Detection limit（S/N=3） was 0.033 μ g/mL, and the limit of quantification（S/ N= 10） was 0.11 μg / mL. This method is suitable for determining the content of rhodamine B in glitter powder.