| 高效液相色谱-质谱联用法测定人体血液中脂肪酸含量 |
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| 引用本文： | 许倩,盛灵慧,李保山,黄峥.高效液相色谱-质谱联用法测定人体血液中脂肪酸含量[J].化学分析计量,2011,20(5):14-16. |
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| 作者姓名： | 许倩 盛灵慧 李保山 黄峥 |
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| 作者单位： | 1. 北京化工大学化工资源有效利用国家重点实验室,北京,100029
2. 中国计量科学研究院,北京,100013 |
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| 基金项目： | 中国计量科学研究院基本科研业务费项目 |
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| 摘 要： | 建立了一种用液相色谱-串联质谱(HPLC- MS)快速、准确地对人体血液中游离脂肪酸进行分离和定量的方法.该法使用Dole提取法,提取的样品直接进行分析,无需衍生化处理.该方法以氘代十六烷酸为内标物,采用液相色谱-质谱联用法测定了人血清中游离脂肪酸的含量,各脂肪酸线性方程的相关系数均大于0.99,方法的回收率为90.0...
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| 关 键 词： | 血清 游离脂肪酸 液相色谱-串联质谱 |
DETERMINATION OF FREE FATTY ACIDS IN HUMAN SERUM BY HPLC-MS |
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| Xu Qian,Sheng Linghui,Li Baoshan,Huang Zheng.DETERMINATION OF FREE FATTY ACIDS IN HUMAN SERUM BY HPLC-MS[J].Chemical Analysis And Meterage,2011,20(5):14-16. |
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| Authors: | Xu Qian Sheng Linghui Li Baoshan Huang Zheng |
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| Institution: | , Huang Zheng( 1. State Key Laboratory of Chemical Resource Engineering, Bejing University of Chemical Technology, Bejing 100029, China; 2. ational Institute of Metrology P. R. China, Beijing 100031, China) |
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| Abstract: | A rapid and sensitive method for separation and quantitation of free fatty acids （FFAs） in human serum by high - performance liquid chromatographymass spectrometry （HPLC- MS） was established. The samples were extracted by Dole method, and injected directly to the HPLC system without derivative treatment. The linear correlation coefficient was more than 0.99, and the RSD of determination results was less than 9% （ n =9）. The recoveries ranged from 90.02% to 100.05%. |
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| Keywords: | serum free fatty acid HPLC - MS |
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