Fluorescent oligonucleotide incorporating 5-(1-ethynylpyrenyl)-2-deoxyuridine: sequence-specific fluorescence changes upon duplex formation |
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| Authors: | Gil Tae Hwang |
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| Affiliation: | National Research Laboratory, Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea |
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| Abstract: | We describe the design and properties of a pyrene-labeled deoxyuridine that can be inserted efficiently into oligodeoxynucleotides using phosphoramidite chemistry. An oligonucleotide incorporating the pyrene-labeled deoxyuridine is a sensitive fluorescence probe that can discriminate between perfect and single-base-mismatched pairing by changes in its fluorescence intensity. |
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| Keywords: | Fluorescence Nucleosides Nucleotides |
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