基于聚乙烯醇离子液体负载HRP修饰石墨烯/纳米金复合膜的过氧化氢生物传感器

以石墨烯/纳米金修饰玻碳电极为基底, 用聚乙烯醇与离子液体复合物将辣根过氧化物酶固定于电极表面, 制备了过氧化氢生物传感器. 结果表明, 在0.1 mol/L HAc-NaAc+0.1mol/L KCl(pH=6.5)中, H₂O₂的氧化峰电流与其浓度在9.55×10^-6~6.01×10^-3 mol/L间呈良好线性关系, 检出限(3S/N)为3.3×10^-7 mol/L. 用标准加入法做回收实验, 回收率在93.4%~100.5%之间. 该传感器对H₂O₂具有较高的灵敏度和较低的检测限, 稳定性和重现性良好, 使用寿命较长, 且制作成本低, 可多次重复使用.

Detection of H2O2 at Composite Membrane of [bmim] FeCl4 and PVA Loading HRP Modified Graphene/nano Au

Polyvinyl alcohol-ionic liquids(PVA-ILs) composite sol, graphenes(GS) and nano-Au were used to immobilize horseradish peroxidase(HRP). A novel hydrogen peroxide biosensor(HRP/PVA-ILs/HRP/nano Au-GS/GC) was obtained by immobilizing HRP with composite membrane of PVA-ILs on a glassy carbon(GC) electrode, which was modified with graphenes and nano Au. The experimental condition was optimized and the electrochemistry behavior of the HRP/PVA-ILs/HRP/nano Au-GS/GC biosensor in 0.1 mol/L HAc-NaAc+0.1 mol/L KCl(pH=6.5) including H₂O₂ was discussed. The response of the sensor to H₂O₂ is linear in the range of 9.55×10^-6-6.01×10^-3 mol/L with a correlation coefficient of 0.9990. The detection limit is 3.3×10^-7 mol/L. The experiments show that the biosensor exhibits high sensitivity, good reproducibility and stability and longer service life.