| 甲烷氧化菌素功能化金纳米层层自组装修饰电极上过氧化氢的催化还原 |
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| 引用本文： | 窦博鑫,辛嘉英,王振兴,王艳,夏春谷.甲烷氧化菌素功能化金纳米层层自组装修饰电极上过氧化氢的催化还原[J].分子催化,2017,31(6):534-543. |
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| 作者姓名： | 窦博鑫 辛嘉英 王振兴 王艳 夏春谷 |
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| 作者单位： | 哈尔滨商业大学 食品科学与工程重点实验室, 黑龙江 哈尔滨 150076,哈尔滨商业大学 食品科学与工程重点实验室, 黑龙江 哈尔滨 150076;中国科学院兰州化学物理研究所 羰基合成与选择氧化国家重点实验室, 甘肃 兰州 730000,哈尔滨商业大学 食品科学与工程重点实验室, 黑龙江 哈尔滨 150076,哈尔滨商业大学 食品科学与工程重点实验室, 黑龙江 哈尔滨 150076,中国科学院兰州化学物理研究所 羰基合成与选择氧化国家重点实验室, 甘肃 兰州 730000 |
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| 基金项目： | 国家自然科学基金（21573055；21073050）；哈尔滨商业大学研究团队支撑项目（2016TD006）；国家重点实验室项目合作项目（cre-2015-c-104） |
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| 摘 要： | 借助巯基试剂,在纳米金颗粒表面修饰生物活性物质Mb,制备保持有Mb生物活性的功能化金纳米巯基乙胺-Au NPs-Mb.采用UV-Vis、FTIR光谱和投射电镜表征其结构,该纳米颗粒分布均匀且粒径均一,并显著改善了金纳米颗粒团聚现象.以Mb功能化金纳米为基元,采用单层自组装及层层自组装方式将其修饰到裸金电极表面.各Mb或Mb-Cu电极的电化学测试并未借助电子传递媒介.配位Cu~(2+)后,修饰有Mb的单层及层层自组装修饰的催化还原能力均显著提升.其中Cu~(2+)配位的{巯基乙胺-Au NPs-Mb}3/Au修饰电极作为一种新型H2O2生物传感器,响应时间大约为2 s,米氏常数KappM为0.787 mmol/L,表现出了较强的还原H2O2的催化活性,且稳定性较好.
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| 关 键 词： | 甲烷氧化菌素 直接电化学 金纳米 自组装膜 过氧化氢 |
| 收稿时间： | 2017/10/3 0:00:00 |
| 修稿时间： | 2017/11/1 0:00:00 |
Multilayer Self-assembly Modified Electrode of Methanobactin Functionalized Gold Nanoparticle to Catalytic Reduction of Hydrogen Peroxide |
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| DOU Bo-xin,XIN Jia-ying,WANG Zhen-xing,WANG Yan and XIA Chun-gu.Multilayer Self-assembly Modified Electrode of Methanobactin Functionalized Gold Nanoparticle to Catalytic Reduction of Hydrogen Peroxide[J].Journal of Molecular Catalysis (China),2017,31(6):534-543. |
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| Authors: | DOU Bo-xin XIN Jia-ying WANG Zhen-xing WANG Yan and XIA Chun-gu |
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| Institution: | Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China,Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China;State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China,Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China,Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China and State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China |
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| Abstract: | Mathanobatins (Mb,Mbtins) were immobilized successfully on nanometer-sized gold colloid particles associated with β-mercaptoethylamine.The structures of Mb functionalized gold nanoparticles were characterized and confirmed by UV-vis spectroscopy (UV-vis),FTIR spectra and Transmission electron microscopy.The nanoparticles with equal distribution and uniform particle size improved reunion phenomenon significantly.Mb functionalized gold nanoparticles as primitives,which was modified on the surface of bare gold electrode by the monolayer self-assembly and multilayer self-assembly.Direct electron transfer between Mb or copper-loading Mbtins and the modified electrode was investigated without the aid of any electron mediator.The copper-loading Mbtins act as a better electrocatalyst for the reduction of H2O2 than Mb.The { mercaptoethylamine-AuNPs-Mb}3 modified gold electrode of copper-loadeding Mb,with which gold nanoparticles were functionalized,as a model enzyme,was immobilized on gold electrode to construct a novel H2O2 biosensor.Response time was about 2 s.The Michaelis-Menten constant (Km) was 0.787 mmol/L.Good stability and sensitivity were assessed for the biosensor. |
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| Keywords: | methanobactin direct electrochemistry gold nanoparticles self-assembled monolayer hydrogen peroxide |
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