| Microcalorimetric studies on the dismutation of superoxide anion catalyzed by superoxide dismutase |
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| 引用本文： | 梁毅,屈松生,汪存信,刘欲文,王志勇,宋昭华,邹国林. Microcalorimetric studies on the dismutation of superoxide anion catalyzed by superoxide dismutase[J]. 中国科学B辑(英文版), 1998, 41(6): 575-586. DOI: 10.1007/BF02883019 |
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| 作者姓名： | 梁毅 屈松生 汪存信 刘欲文 王志勇 宋昭华 邹国林 |
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| 作者单位： | LIANG Yi，QU Songsheng，WANG Cunxin LIU Yuwen，WANG Zhiyong，SONG Zhaohua (Department of Chemistry，Wuhan University，Wuhan 430072，China)and ZOU Guolin (College of Life Science，Wuhan University，Wuhan 430072，China) |
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| 基金项目： | ProjectsupportedbytheNationalNaturalScienceFoundationofChina (GrantNo .2 92 7312 1) |
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| 摘 要： | Ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ(ＳＯＤ,ＥＣ1.15.1.1),ｗｈｉｃｈｗａｓｆｏｕｎｄａｎｄｉｓｏｌａｔｅｄｆｒｏｍｂｏｖｉｎｅｅｒｙｔｈｒｏｃｙｔｅｓｂｙＭｃＣｏｒｄａｎｄＦｒｉｄｏｖｉｃｈｉｎ1969[1],ｉｓａｎｉｍｐｏｒｔａｎｔｍｅｍｂｅｒｏｆｔｈｅｆａｍｉｌｙｏｆｂｉｏｌｏｇｉｃａｌａｎｔｉｏｘｉｄａｎｔｓｔｒｅｓｓｅｎｚｙｍｅｓ.Ｔｈｉｓｅｎｚｙｍｅｈａｓｂｅｅｎｄｅｔｅｃｔｅｄｉｎａｗｉｄｅｒａｎｇｅｏｆｌｉｖｉｎｇｔｈｉｎｇｓａｎｄｈａｓｂｅｅｎｉｍｐｌｉｃａｔｅｄｉｎｔｈｅｉｎｔｅｒ…
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| 收稿时间： | 1998-03-01 |
Microcalorimetric studies on the dismutation of superoxide anion catalyzed by superoxide dismutase |
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| Yi Liang,Songsheng Qu,Cunxin Wang,Yuwen Liu,Zhiyong Wang,Zhaohua Song,Guolin Zou. Microcalorimetric studies on the dismutation of superoxide anion catalyzed by superoxide dismutase[J]. Science in China(Chemistry), 1998, 41(6): 575-586. DOI: 10.1007/BF02883019 |
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| Authors: | Yi Liang Songsheng Qu Cunxin Wang Yuwen Liu Zhiyong Wang Zhaohua Song Guolin Zou |
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| Affiliation: | (1) Department of Chemistry, Wuhan University, 430072 Wuhan, China;(2) College of Life Science, Wuhan University, 430072 Wuhan, China |
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| Abstract: | Thermokinetics of the dismutation of superoxide anion (O 2 .- ) catalyzed by superoxide dismutase (SOD), in which the decomposition of hydrogen peroxide catalyzed by catalase is utilized as a source of oxygen and the autoxidation of pyrogallod as a source of the substrate (O 2 .- ), has been studied by microcalorimetry. The molar reaction enthalpies of the SOD reaction and the pyrogallol autoxidation have been measured as - 160.1 and - 218 kJ · mol-1, respectively, at 298. 15 K and pH 7.0. A novel method for the determination of SOD activity, the microcalorimetric assay for SOD, has been proposed. The experimental results showed that SOD had no effect on both kinetic parameters and the mechanism for the pyrogallol autoxidation. This autoxidation followed the second-order reaction kinetics in the presence of limited oxygen (the first order with respect to both pyrogallol and O2), and the second-order rate constants were hermined at 298.15 K and pH 8.0 to be 1.25 and 1.30 L·mol-1·s-1 in the absence and presence of SOD, respectively. A possible mechanism for the autoxidation of pyrogallol inhibited by SOD was also suggested. Project supported by the National Natural Science Foundation of China (Grant No. 29273121). |
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| Keywords: | microcalorimetry superoxide dismutase pyrogallol autoxidation thermokinetics kinetics reaction mechanism. |
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