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Rapid and efficient method for the quantification of lychnopholide in rat plasma by liquid chromatography–tandem mass spectrometry for pharmacokinetic application |
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| Authors: | Larissa Lachi‐Silva João Paulo Barreto Sousa Maiara Camotti Montanha Sherwin K. B. Sy João Luis Callegari Lopes Denise Brentan Silva Norberto Peporine Lopes Andréa Diniz Elza Kimura |
| Affiliation: | 1. Preclinical Pharmacokinetic Laboratory, Pharmacy Department, Maringa State University, Maringá–, PR, Brazil;2. Núcleo de Pesquisa em Produtos Naturais e Sintéticos, Physics and Chemistry Department, College of Pharmaceutical Sciences of Ribeir?o Preto, University of S?o Paulo, Ribeir?o Preto–, SP, Brazil;3. Núcleo de Pesquisa Clínica e Bioequivalência, University Hospital, Maringa State University, Maringá–, PR, Brazil;4. Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville–, Florida, USA;5. Biostatistics Graduate Program, Maringa State University, Maringá–, PR, Brazil;6. Laboratório de Produtos Naturais e Espectrometria de Massas, Center of Biological and Health Sciences, Mato Grosso do Sul Federal University, Campo Grande–, MS, Brazil |
| Abstract: | Lychnopholide is a sesquiterpene lactone usually obtained from Lychnophora and Eremanthus species and has pharmacological activities that include anti‐inflammatory and anti‐tumor. Lychnopholide isolated from Eremanthus matogrossenssis was analyzed in this study. The aims of this study were to develop and validate an analytical methodology by LC‐MS/MS and to quantify lychnopholide in rat plasma. Chromatographic separation was achieved on a C18 column using isocratic elution with the mobile phase consisting of methanol and water (containing 0.1% formic acid) at a flow rate of 0.4 mL/min. The detection was performed in multiple‐reaction monitoring mode using electrospray ionization in positive mode. The method validation was performed in accordance with regulatory guidelines and the results met the acceptance criteria. The linear range of detection was 10–200 ng/mL (r > 0.9961). The intra‐ and inter‐day assay variability were <6.2 and <11.7%, respectively. The extraction recovery was approximately 63% using liquid–liquid extraction with chloroform. Lychnopholide was detected in plasma up to 60 min after intravenous administration in rats. This rapid and sensitive method for the analysis of the sesquiterpene lactone lychnopholide in rat plasma can be applied to pharmacokinetic studies of this compound. Copyright © 2016 John Wiley & Sons, Ltd. |
| Keywords: | lychnopholide sesquiterpene lactone LC‐MS/MS pharmacokinetics |