| Retention Behaviors of Uronic Acid-containing Polysaccharides and Neutral Polysaccharides in HPGPC |
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| 作者姓名： | Cui Ping LIU Xing Feng BAO Ji Nian FANG* Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai |
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| 作者单位： | Cui Ping LIU，Xing Feng BAO，Ji Nian FANG* Shanghai Institute of Materia Medica，Shanghai Institutes for Biological Sciences，Chinese Academy of Sciences，Shanghai 200031 |
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| 摘 要： | The determination of molecular weights (Mw) of polysaccharides has served as an important process since the physical properties of the polysaccharides are closely related to their Mw and/or molecular weights distribution (MWD). For Mw and MWD determinations, high performance gel permeation chromatography (HPGPC) has gained wide acceptance as a preferred method due to its high sensitivity, speed and reproducibility. In chromatography, it is well known that the molecular weights are determ…
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Retention Behaviors of Uronic Acid-containing Polysaccharides and Neutral Polysaccharides in HPGPC |
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| Cui Ping LIU,Xing Feng BAO,Ji Nian FANG* Shanghai Institute of Materia Medica,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai .Retention Behaviors of Uronic Acid-containing Polysaccharides and Neutral Polysaccharides in HPGPC[J].Chinese Chemical Letters,2001(10). |
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| Authors: | Cui Ping LIU Xing Feng BAO Ji Nian FANG |
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| Institution: | Cui Ping LIU,Xing Feng BAO,Ji Nian FANG* Shanghai Institute of Materia Medica,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031 |
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| Abstract: | The chromatographic behaviors of several uronic acid-containing polysaccharides and neutral polysaccharides were investigated in HPGPC for the first time. The effects of sample concentration and ionic strength of mobile phase on retention time were studied. The mechanism for the effects on Mw determination results of polysaccharides by HPGPC was also discussed. |
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| Keywords: | Polysaccharides HPGPC molecular weights |
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