| 3,4,5-三羟基苯甲酸通过线粒体途径诱导人肝癌细胞SMMC-7721的凋亡 |
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| 引用本文： | 赵文静,牛凤兰.3,4,5-三羟基苯甲酸通过线粒体途径诱导人肝癌细胞SMMC-7721的凋亡[J].高等学校化学学报,2009,30(2):320-323. |
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| 作者姓名： | 赵文静 牛凤兰 |
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| 作者单位： | 吉林大学公共卫生学院,长春,130021 |
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| 基金项目： | 吉林省科技厅重点项目 |
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| 摘 要： | 采用四甲基偶氮唑盐(MTT)观察3,4,5-三羟基苯甲酸(TBA)对人肝癌SMMC-7721细胞的增殖抑制作用; 通过流式细胞仪检测细胞凋亡、细胞内活性氧(ROS)及线粒体膜电位的变化, 用比色法测定Caspase-9和Caspase-3蛋白活性, 探讨TBA 诱导SMMC-7721细胞凋亡的分子机制. 研究结果表明, TBA对SMMC-7721细胞生长具有显著的抑制作用并诱导其凋亡, 诱导SMMC-7721细胞凋亡作用可能通过线粒体信号传导通路实现.
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| 关 键 词： | 3 4 5-三羟基苯甲酸 人肝癌SMMC-7721细胞 凋亡 线粒体膜电位 |
| 收稿时间： | 2008-05-05 |
Trihydroxybenzoic Acid on Induction of the Apoptosis of Human Hepatocarcinoma SMMC-7721 Cells by Mitochondrial-dependent Pathway |
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| ZHAO Wen-jing,NIU Feng-lan.Trihydroxybenzoic Acid on Induction of the Apoptosis of Human Hepatocarcinoma SMMC-7721 Cells by Mitochondrial-dependent Pathway[J].Chemical Research In Chinese Universities,2009,30(2):320-323. |
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| Authors: | ZHAO Wen-jing NIU Feng-lan |
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| Institution: | School of Public Health;Jilin University;Changchun 130021;China |
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| Abstract: | Trihydroxybenzoic acid show the anti-tumor potency from many previous investigations,but the research for the inhibitory effect and mechanism on SMMC-7721 cells has not been regarded so far.MTT assay was used to evaluate the anti-proliferation effect of trihydroxybenzoic acid on SMMC-7721 cells and human PBMC.ROS production,apoptotic cells and the mitochondrial membrane potential were detected by flow cytometry.The activities of Caspase-3,9 were measured by colorimetric method.The results show that trihydro... |
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| Keywords: | Trihydroxybenzoic acid Human hepatocarcinoma SMMC-7721 cells Apoptosis Mitochondrial membrane potential |
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