| 无筛板型固相萃取柱的制备及其在食品中苯甲酸的测定研究 |
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| 引用本文： | 宋文青,刘亚雄,王琴,包建民.无筛板型固相萃取柱的制备及其在食品中苯甲酸的测定研究[J].分析化学,2009,37(10). |
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| 作者姓名： | 宋文青 刘亚雄 王琴 包建民 |
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| 作者单位： | 1. 天津大学药物科学与技术学院,天津,300072
2. 天津奥秘科技有限公司,天津,300284 |
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| 基金项目： | 天津市科技发展计划项目 |
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| 摘 要： | 结合固相萃取(SPE)盘与含支撑物的SPE柱技术,制备了一种新型的无筛板型固相萃取柱.以C18填料为例,以话梅样品为介质对其中的苯甲酸进行分析,并用传统固相萃取小柱平行比较;将SPE与HPLC-UV结合,考察了填料对简单介质中苯甲酸的最大吸附量及洗脱曲线,研究了新型SPE柱在实际应用中的分离纯化效果.结果表明,新型SPE柱对样品的吸附效果更好,规格为200 mg/3 mL的SPE柱对苯甲酸的吸附量达到0.951 mg,超过了传统柱的吸附量0.908 mg;其洗脱曲线与传统柱几乎重合;苯甲酸在1～100 mg/L浓度范围内线性关系良好, r=0.9999,用此SPE柱纯化后的样品加标回收率和相对误差分别在88.4%～102.3%和1.4%～2.9%之间.
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| 关 键 词： | 固相萃取柱 筛板 填料 苯甲酸 |
Preparation of Novel Stationary Phase for Solid Phase Extraction and its Application for the Analysis of Benzoic Acid in Plum |
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| SONG Wen-Qing,LIU Ya-Xiong,Wang Qin,BAO Jian-Min.Preparation of Novel Stationary Phase for Solid Phase Extraction and its Application for the Analysis of Benzoic Acid in Plum[J].Chinese Journal of Analytical Chemistry,2009,37(10). |
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| Authors: | SONG Wen-Qing LIU Ya-Xiong Wang Qin BAO Jian-Min |
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| Institution: | SONG Wen-Qing1,LIU Ya-Xiong1,Wang Qin2,BAO Jian-Min11(School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072)2(Aumist Technology Co.,Ltd.,Tianjin 300284) |
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| Abstract: | Based on technologies of solid phase extraction(SPE) cartridge and SPE disc,a novel SPE cartridge of no frits was prepared.Taking C18 as sample,in comparison with traditional packing,the performance of SPE cartridge was evaluated by benzoic acid in plum sample.The saturation adsorption of benzoic acid by two kinds of SPE and their elution curves were tested by HPLC-UV with solid phase extraction,and the capability of novel sorbent in application was examined.The results showed that the new format SPE had be... |
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| Keywords: | Solid phase extraction cartridge frits sorbent benzoic acid |
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