Separation of chlamydosporol epimers by reversed-phase HPLC using commercial solvent optimisation software |
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| Authors: | M Solfrizzo M Fobes-Smith R N Strange A Visconti |
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| Institution: | (1) Istituto Tossine e Micotossine da Parassiti Vegetali, C.N.R., Viale L. Einaudi 51, 70125 Bari, Italy;(2) Department of Biology, Darwin Building, University College London, Gower Street, WC1E 6BT London, UK |
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| Abstract: | Summary Two epimers of the mycotoxin chlamydosporol were separated by HPLC on an RP-18 column using a quaternary mobile phase consisting of water (79.1%), methanol (10.0%), acetonitrile (10.4%) and tetrahydrofuran (0.5%), with a flow rate of 1 ml min–1. This optimal composition of mobile phase, with which the resolution value for the two epimers (1 and2) was 2.73 with retention times of 5.88 and 7.12 min, respectively, was achieved by the application of Philips Solvent Optimisation Software PU 6100. The presence of free silanols on the stationary phase was shown to be an essential requirement for the separation of the chlamydosporol epimers. |
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| Keywords: | Column liquid chromatography Mycotoxins Chlamydosporol epimers Solvent optimisation Fusarium species |
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