Electrochemical enzymatic determinations of ethanol and l-lactic acid with a carbon paste electrode modified chemically with nicotinamide adenine dinucleotide |
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| Authors: | Toshio Yao Soichiro Musha |
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| Institution: | Department of Applied Chemistry, College of Engineering, University of Osaka Prefecture, Mozu-umemachi, Sakai 591 Japan |
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| Abstract: | The oxidized form of nicotinamide adenine dinucleotide (NAD+) is chemically immobilized at the surface of a carbon paste electrode containing n-octaldehyde. The NAD+ is converted to NADH by oxidation of ethanol and l-lactic acid catalyzed by their respective dehydrogenases, and the NADH formed is oxidized electrochemically to the original NAD+, thus giving a well defined linear-sweep voltammetric peak. The peak area is linearly related to the amount of ethanol or l-lactic acid in the range 0.05–2 × 10-9 mol. |
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