多巴胺有序介孔硅表面分子印迹传感器的研制

以多巴胺(DA)为模板,氨基修饰的介孔硅为载体,制得对多巴胺具有特异选择性的表面分子印迹聚合物(MIP)。将所得的MIP制成碳糊电极,用循环伏安法对多巴胺进行检测。在优化实验条件下,传感器的氧化峰电流与多巴胺浓度在1.0×10-7～2.0×10-6mol/L和2.0×10-6～1.0×10-4mol/L范围内呈良好的线性关系,相关系数分别为0.992 5和0.996 9,检出限为1.3×10-9mol/L。该传感器对DA具有较高的灵敏度和选择性,将其用于实际样品检测,结果满意。

Preparation of Sensor for Dopamine Based on Surface Molecularly Imprinted Ordered Mesoporous Silica

A molecularly imprinted polymer(MIP),which has a specific selectivity to dopamine,was synthesized using dopamine as template and 3-aminopropyltriethoxysilane modified mesoporous silica as carrier.The MIP was used to fabricate a sensor for the determination of dopamine,which response to dopamine(DA) was evaluated by cyclic voltammetry.Under the optimized conditions,the sensor displayed good linearities in the ranges of 1.0×10-7-2.0×10-6 mol/L and 2.0×10-6-1.0×10-4 mol/L with a lower detection limit of 1.3×10-9 mol/L.The correlation coefficients were 0.992 5 and 0.996 9,respectively.The studied imprinted sensor exhibited high sensitivity and selectivity,and was successfully applied in the determination of dopamine in human blood plasma.