Immunocytochemical and structural comparative study of committed versus multipotent stem cells cultured with different biomaterials |
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| Institution: | 1. Department of Biomedical Sciences, Section of Human Morphology, University of Modena and Reggio Emilia, Italy;2. Department of Mechanical and Civil Engineering, University of Modena and Reggio Emilia, Italy;1. Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Bellaterra, Barcelona, 08193, Spain;2. Departament Biologia Cel·lular, Fisiologia i Immunologia, Facultat Biociències, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, 08193, Spain;3. Department of Chemical Engineering & Materials Science, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA;4. Instituto de Ciencia de Materiales de Madrid (ICMM-CSIC), Madrid, 28049, Spain;5. Department of Electrical & Computer Engineering, University of Minnesota Twin Cities, Minneapolis, MN 55455, USA;1. Departament de Física, Universitat Autònoma de Barcelona, E-08193, Bellaterra, Cerdanyola del Vallès, Spain;2. Departament de Biologia Cellular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, E-08193, Bellaterra, Cerdanyola del Vallès, Spain;3. ICREA, Pg. Lluís Companys 23, E-08010, Barcelona, Spain;1. Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, S.S. dell’Abetone e del Brennero 4, 56123 Pisa, Italy;2. Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M.Aleandri”, Via Appia Nuova 1411, 00178 Roma, Italy;3. ARPAT, Agenzia Regionale per la Protezione Ambientale Toscana, Via Marrani 114, 57126 Livorno, Italy;4. Università degli Studi di Teramo, Facoltà di Medicina Veterinaria, Località Piano d’Accio, 64100 Teramo, Italy;1. College of Materials and Textile Engineering, Jiaxing University, Jiaxing, 314001, China;2. Key Laboratory of Yarn Materials Forming and Composite Processing Technology of Zhejiang Province, Jiaxing University, Jiaxing, 314001, China;1. Dpto. Física de Materiales, Facultad de Ciencias Físicas, Universidad Complutense de Madrid, Ciudad Universitaria s/n, Madrid 28040, Spain;2. Institute for Optoelectronic Systems and Microtechnology (ISOM), Polytechnic University of Madrid (UPM), Avenida Complutense 30, Madrid 28040, Spain;3. Instituto de Magnetismo Aplicado, UCM-ADIF-CSIC, Las Rozas, Spain |
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| Abstract: | The aim of this work was the comparison of the behavior of committed (human osteoblast cells – hOB – from bone biopsies) versus multipotent (human dental pulp stem cells – hDPSC – from extracted teeth) cells, cultured on shot-peened titanium surfaces, since the kind of cell model considered has been shown to be relevant in techniques widely used in studies on composition/morphology of biomaterial surfaces. The titanium surface morphology, with different roughness, and the behavior of cells were analyzed by confocal microscope (CM), scanning electron microscope (SEM) and X-ray microanalysis. The best results, in terms of hOB adhesion/distribution, were highlighted by both CM and SEM in cultured plates having 20-μm-depth cavities. On the contrary, CM and SEM results highlighted the hDPSC growth regardless the different surface morphology, arranged in overlapped layers due to their high proliferation rate, showing their unfitness in biomaterial surface test. Nevertheless, hDPSC cultured inside 3D-matrices reproduced an osteocyte-like three-dimensional network, potentially useful in the repair of critical size bone defects. The behavior of the two cell models suggests a different use in biomaterial cell cultures: committed osteoblast cells could be appropriate in selecting the best surfaces to improve osseointegration, while multipotent cells could be suitable to obtain in vitro osteocyte-like network for regenerative medicine. The originality of the present work consists in studying for the first time two different cell models (committed versus multipotent) compared in parallel different biomaterial cultures, thus suggesting distinct targets for each cellular model. |
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