Rational redesign of Candida antarctica lipase B for the ring opening polymerization of D,D-lactide |
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| Authors: | Takwa Mohamad Larsen Marianne Wittrup Hult Karl Martinelle Mats |
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| Institution: | Royal Institute of Technology, School of Biotechnology, Department of Biochemistry, AlbaNova University Centre, 106 91 Stockholm, Sweden. |
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| Abstract: | Based on molecular modelling, the enzyme Candida antarctica lipase B was redesigned as a catalyst for the ring opening polymerization of D,D-lactide. Two mutants with 90-fold increased activity as compared to the wild-type enzyme were created. In a preparative synthesis of poly(D,D-lactide) the mutants greatly improved the rate and the degree of polymerization. |
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