海洋珍珠生物提取液对人宫颈癌Siha细胞增殖与凋亡作用的影响

为探讨海洋珍珠生物提取液对宫颈癌Siha细胞株增殖和凋亡的影响,采用MTT法检测了细胞增殖情况,在流式细胞仪用Annexin V和PI双染检测了细胞的凋亡率,PI单染法测定了细胞周期,Hoechst 33258/PI荧光染色法观测了Siha细胞的形态学改变,RT-PCR法测定了宫颈癌Siha细胞株内Bcl-2,Bax基因表达。结果表明,海洋珍珠生物提取液在一定质量浓度范围内,以浓度依赖性的方式抑制宫颈癌Siha细胞的生长(P0.05);以0,6,30和60μg/mL海洋珍珠生物提取液处理细胞24 h,Siha细胞早期凋亡率分别为5%,7.1%,32.25%和31.95%,晚期凋亡比例为0.45%,2.85%,8.55%和23.2%;荧光显微镜下细胞呈现典型的凋亡性改变;RT-PCR显示海洋珍珠生物提取液处理后宫颈癌Siha细胞内的Bcl-2 mRNA表达降低,Bax mRNA表达升高。提示海洋珍珠生物提取液以浓度依赖性的方式抑制宫颈癌Siha细胞增殖,并通过降低Bcl-2 mRNA,升高Bax mRNA来诱导细胞凋亡。

Effect of Extraction of Pelagi - Margarita on Proliferation and Apoptosis of Human Cervical Cancer Cell Line Siha

To explore the effect of extraction of pelagi - margarita （EPM） on the apoptosis of cervical cancer Siha cell line, the growth inhibition rate of Siha cells was detected by MTT method, and cell apoptosis rate was analyzed by flow cytometry （FCM） using annexinV/propidium iodide （PI） double staining, cell cycle was detected by flow cytometry using PI staining, morphologic changes of apoptotic cells were observed by fluorescence microscope, expression of intracellular Bcl - 2, Bax protein was analyzed by real time PCR. The results showed that EPM inhibited the proliferation of Siha cells in a dose - dependent manner（P 〈 0. 05）. After EPM treatment at 0, 6, 30 and 60 μg/mL for 24 h, the apoptosis rate of Siha cells was 5%, 7.1%, 32.25% and 31.95%, respectively, while the necrosis rate was 0. 9%, 1.55%, 2. 55% and 40. 05%. The cells showed characteristic apoptotic morphologic changes under fluorescence microscope. Bioenergy vigor at various concentration could down - regulate the expression of Bcl - 2 mRNA, meanwhile, up - regulate the expression of Bax mRNA. It concludes EPM inhibits the proliferation in a dose - dependent manner, and induces theapoptosis of Siha cells by down- regulate the expression of Bcl - 2 mRNA, meanwhile, up - regulate the expression of Bax mRNA.