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Highly sensitive determination of doxorubicin and daunorubicin based on their effect on the resonance light scattering signals of the ethidium-DNA complex |
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| Authors: | Zhanguang Chen Sihua Qian Guoliang Liu Xi Chen Junhui Chen |
| Institution: | 1. Department of Chemistry, Shantou University, Shantou, 515063, China 2. Guangdong Pharmaceutical University, Guangzhou, 510006, China 3. Department of Radiology, Peking University Shenzhen Hospital, Shenzhen, 518036, China |
| Abstract: | We have developed a resonance light scattering (RLS) quenching assay for the highly sensitive determination of doxorubicin (DOX) and daunorubicin (DAU). It is based on the reduction of the intensity of the shoulder of the RLS spectra at 443?nm. The intensity of the RLS of the ethidium-DNA system decrease linearly on addition of trace quantities of DOX or DAU within the concentration range of 0.008 to 12.0???g?mL?1 for DOX, and of 0.010 to 21.0???g?mL?1 for DAU. The detection limits are 3.0 and 5.0?ng?mL?1, respectively. The assay was successfully applied to the determination of DAU in synthetic and serum samples. Compared to the reported methods for anthracyclines, this assay displays higher sensitivity, lower detection limits, and a wider linear range. The addition of trace amount of drugs into the EB-DNA system can induce the decreased RLS intensity of EB-DNA system at the shoulder peak in BR buffer solution (pH 2.0). Besides, the decrement of RLS intensities was proportional to the concentration of drugs. Based on this phenomenon, a new RLS assay for the detection of anthracycline antibiotics was developed. |
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