The role of ester groups in resistance of plant cell wall polysaccharides to enzymatic hydrolysis |
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Authors: | Grohmann K Mitchell D J Himmel M E Dale B E Schroeder H A |
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Institution: | 1.Solar Fuels Research Diu., Biotechnology Branch, Solar Energy Research Institute, 80401, Golden, CO ;2.Colorado State University, CO, Fort Collins, 80523 ; |
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Abstract: | Xylan backbones in native plant cell walls are extensively acety-lated. Previously, no direct investigations as to their role
in cellulolytic enzyme resistance have been done, though indirect results point to their importance. An in vitro deesterification
of aspen wood and wheat straw has been completed using hydroxylamine solutions. Yields of 90% acetyl ester removal for both
materials have been accomplished, with little disruption of other fractions (i.e., lignin). Apparently, as the xylan becomes
increasingly deacetylated, it becomes 5–7 times more digestible. This renders the cellulose fraction more accessible, and
2–3 times more digestible. This effect levels off near an acetyl removal of 75%, where other resistances become limiting. |
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Keywords: | Index Entries" target="_blank">Index Entries Xylan ester groups cellulase pretreatment |
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