Synthetic surfaces for ribonuclease adsorption |
| |
| Authors: | Smith Bryan D Soellner Matthew B Raines Ronald T |
| |
| Institution: | Department of Biochemistry and Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA. |
| |
| Abstract: | Intact RNA and DNA are of central importance to biochemical research and biotechnology. The preservation of these nucleic acids requires the absence of nuclease activity. Here, radical-mediated polymerization of vinylsulfonate on resin and glass surfaces is shown to produce a high-density poly(vinylsulfonate) coating that sequesters ribonucleases from aqueous solutions quickly and completely. The adsorptive efficacy of this coating exceeds that of other known coatings by > or =10(7)-fold. Surfaces coated with poly(vinylsulfonate) could be used to maintain the integrity of ribonucleic acids in a variety of contexts. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
