Study on the interaction between methyl violet and bovine serum albumin by spectral analyses |
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Authors: | Yanyan Hu Suqin Xu Xiashi Zhu Aiqin Gong |
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Institution: | aDepartment of Chemistry, Yangzhou University, Yangzhou 225002, Jiangsu, PR China |
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Abstract: | In this article the interaction between methyl violet (MV) and bovine serum albumin (BSA) was studied with spectroscopy. The results indicated that the fluorescence intensity of BSA was quenched strongly by MV through a static quenching procedure. The association constants, the number of binding sites and basic thermodynamic parameters were obtained based on fluorescence quenching data. The effect of MV on the conformation of BSA had been investigated with synchronous fluorescence spectroscopy and circular dichroism (CD) spectrum. |
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Keywords: | Bovine serum albumin Methyl violet Fluorescence spectroscopy Synchronous fluorescence spectroscopy Dichroism spectrum |
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