二茂铁与DNA作用的表面电化学研究

在研究碳纳米管电极上二茂铁电化学性质的基础上,应用二茂铁修饰电极和DNA修饰电极研究了二茂铁与小牛胸腺DNA的相互作用。结果表明,修饰电极上的二茂铁都呈现一对明显的氧化还原峰,二茂铁修饰电极与DNA的作用表现为氧化还原峰电流减小,与溶液中的两者作用情况类似,而DNA修饰电极与二茂铁的作用则表现为氧化还原峰电流增大。扫描电镜结果也证实了两种修饰电极上的二茂铁与DNA间的作用。此外,还讨论了二茂铁与DNA间的作用模式。

Surface Electrochemical Studies of Ferrocene and Its Interaction with DNA

The interaction of ferrocene with calf thymus DNA has been investigated by using the ferrocene and/or DNA modified electrodes based on the electrochemical properties of ferroceneon on the carbon nanotube_modified electrode. The results show that an obvious redox peak for ferrocene is exhibited on the modified electrode. There exists a decrease for the redox peak currents on the electrode modified by ferrocene in the presence of DNA, which is similar to their interaction in solution. However, when the electrode modified by DNA is used as the working electrode, the redox peak current appears an increase compared with that by using the carbon nanotube_modified electrode. The interaction between ferrocene and DNA is also proved by SEM. In addition, the interaction mode of ferrocene with DNA is discussed.