| Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase |
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| 作者姓名： | CUI Wei HUANG Lei LI Wei WANG Li-ping |
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| 作者单位： | CUI Wei，HUANG Lei，LI Wei and WANG Li-ping*College of Life Science，Jilin University，Changchun 130021，P. R. China |
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| 摘 要： | Introduction S Adenosylmethionine(SAM)isabiologicallyac tivecompoundwidelydistributedinthebodytissues andfluids.Itisinvolvedinanumberofbiochemical reactions.Asamethylgroupdonor,itisimportantin transmethylationreactions,whichcontributestothe synthesesofsuc…
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| 关 键 词： | 纯系化 SAM SAM合成酶 S-腺苷甲硫氨酸合成酶 生物活性 cDNA 原核表达 |
| 文章编号： | 1005-9040（2006）-02-236-03 |
| 收稿时间： | 2006-01-10 |
Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase |
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| CUI Wei HUANG Lei LI Wei WANG Li-ping.Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase[J].Chemical Research in Chinese University,2006,22(2):236-238. |
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| Authors: | Wei CUI Lei HUANG Wei LI Li-ping WANG |
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| Institution: | aCollege of Life Science, Jilin University, Changchun 130021, P. R. China |
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| Abstract: | The S-adenosylmethionine synthetase(SAM synthetase) is responsible for in vivo synthesis of S-adenosylmethionine(SAM), which is a kind of biologically active molecules distributed in all body tissues and fluids and involved in a number of biochemical reactions. In this study, a cDNA containing the coding sequence for rat liver SAM synthetase was cloned into the prokaryotic expression vector pQE30 and expressed in E. coli M15. A major band corresponding to a protein of 48 kDa was detected on SDS-PAGE. The protein was distributed in both the soluble fraction and the insoluble fraction. In soluble fractions the protein was fully active. |
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| Keywords: | SAM SAM synthetase Cloning Expression HPLC |
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