Langendorff灌流心脏的31P NMR谱测定

测定了Langendorff灌流大鼠和兔心脏的³¹P NMR谱.观测到PCr、ATP、SP、P_i以及PME和GPC等含磷代谢物的共振峰,各谱峰之间实现了很好的分辨.在3~5min的累加时间内测得的图谱具有较好的S/N,可用于以上含磷代谢物的定量测定,以高能含磷化合物ATP和PCr相对峰强度以及冠脉流量的变化对灌流大鼠心脏代谢的稳定性进行了考察,结果表明,在本实验条件下大鼠心脏的能量储存至少可稳定2h,用所建方法以3min的时间间隔对同一大鼠心脏缺血21min及再灌注12min过程中心脏的³¹P NMR谱进行了连续跟踪测定,并初步观测了缺血及再灌注过程中心肌细胞内ATP、PCr与P_i之间的消长关系.

31P NMR SPECTROSCOPY DETERMINATION OF LANGENDORFF-PERFUSED HEARTS

³¹P NMR spectra of Langendorff-perfused rat and rabbit hearts were measured.Well resolved peaks of PCr, ATP, P_i, PME and GPC Were Observed over 3-5min accumulation times with enough S/N ratios to quantitate these phosphate metabolites.The energy stores of rat hearts were stable for at least 2h as shown by the good stabilities of the peak intensities of ATP and PCr,as well as the coronary flows under our experimental conditions.With the method we established,³¹P NMR spectra of a rat heart during 21 min ischemia and 12min reperfusion were obtained consecutively.The relative Changes between ATP, PCr and P_i Peak intensities were observed over this time course.