Study on the influence of cross-sectional area and zeta potential on separation for hybrid-chip-based capillary electrophoresis using 3-D simulations |
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Authors: | Wu Zeng-Qiang Cao Xiao-Dan Chen Lu Zhang Jian-Rong Xia Xing-Hua Fang Qun Chen Hong-Yuan |
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Institution: | Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China. |
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Abstract: | Hybrid chips combing microchips with capillaries have displayed particular advantages in achieving UV-vis and mass spectroscopic detection. In this work, systematic 3-D numerical simulations have been carried out to explore the influence of junction interface cross-sectional area and ζ-potential distribution on sample band broadening in hybrid-chip electrophoresis separation. In this case, the ratio of cross-sectional area of chip to capillary channel (S(ratio) ) is used as the parameter of the variation in junction interface cross-sectional area. Theoretical simulations demonstrated that the decrease of the S(ratio) would increase the separation efficiency in the hybrid-chip-based CE with uniform ζ-potential distribution. ζ-potential distribution along the axial direction of the channel also affects mass transport in hybrid-chip-based CE. Therefore, the effect of ζ-potential distribution has been considered in the 3-D simulation. Theoretical simulation results reveal that ζ-potential distribution rather than the interface cross-sectional area variation (S(ratio) ) controls the sample band broadening and manipulates sample separation efficiency in the hybrid-chip-based CE with non-uniform ζ-potential distribution. Both the theoretical simulations and experimental results show that optimal hybrid-chip CE separation efficiency can be achieved at S(ratio) =1. |
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