UV-B诱导的大豆愈伤组织超弱光子辐射的动力学分析

为了研究细胞超弱光子辐射的动力学特征及其所揭示的生物学意义，用20 μW/cm2UV-B辐射大豆愈伤组织2 h，测定停止辐射后4 d内大豆愈伤组织在LED光诱导下的延迟发光.通过建立延迟发光动力学方程和数学拟合得到了大豆愈伤组织超弱光子辐射中的延迟发光积分强度、初始光子数、衰减参数和自发发光，讨论了这些发光动力学参数的生物学意义.研究结果表明，在停止UV-B辐射后的4 d内，大豆愈伤组织的光诱导延迟发光服从双曲线弛豫.动力学分析发现，延迟发光积分强度和初始光子数随处理后时间的进行呈现波动变化，停止UV-B辐射后，自发发光和丙二醛（MDA）含量均呈现升高的趋势，在辐射后2 d附近达到峰值，此后同步下降.用延迟发光积分强度和自发发光的比值定义细胞的状态参量Q和序参量R，发现UV-B辐射后大豆愈伤组织细胞Q值或R值的变化反映了UV-B辐射对大豆愈伤组织细胞的损伤以及细胞的恢复过程.

Dynamic Analysis of Ultra-weak Photon Radiation ofSoybean Callus Induced by UV-B Radiation

In order to study the dynamic characteristics of ultra-weak photon emission in cell and its biological significance,soybean callus are radiated by UV-B radiation with 20μW/cm2 for 2 hours.Delayed luminescence of soybean callus induced by light within 4 days after the UV-B radiation is determined.Delayed luminescence integral intensity,the initial photon number,attenuation parameters and the spontaneous luminescence in ultra-weak photon emission of soybean callus are obtained through the establishment of delayed luminescence dynamics equation and mathematical fitting.The biological significance of these dynamic parameters of ultra-weak photon emission is discussed.The results show that delayed luminescence induced by light of soybean callus within 4 days after UV-B radiation to obey the laws of hyperbolic relaxation.Dynamic analysis shows that the changes of the delayed luminescence integral intensity and initial photon number with the time after UV-B treatment are volatility.The intensity of spontaneous luminescence and the content of malondialdehyde(MDA) are increased after the cessation of UV-B radiation.The peaks of them appear nearly two days after UV-B radiation.Using the ratio of delayed luminescence integral intensity and spontaneous luminescence to define the state parameter Q and the order parameter R of cell,it is found that the changes of Q value or R value of soybean callus cells after UV-B radiation reflect the damage of UV-B radiation on soybean callus and cell recovery process.