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Urinary signature of anabolic steroids and glucocorticoids in humans by LC-MS |
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| Authors: | Kiss Agneta Jacquet Anne-Laure Paisse Olivier Flament-Waton Marie-Magdeleine de Ceaurriz Jacques Bordes Claire Gauvrit Jean-Yves Lantéri Pierre Cren-Olivé Cécile |
| Affiliation: | a Service Central d’Analyse (SCA), USR 59 CNRS, Chemin du Canal, Echangeur de Solaize, F-69390 Solaize, France;b Agence Française de Lutte contre le Dopage (AFLD), Département des analyses, 143, avenue Roger Salengro, F-92290 Châtenay-Malabry, France;c Laboratory of Chemometrics, UMR 5180, ESCPE Lyon, University Claude Bernard Lyon 1, Villeurbanne, France |
| Abstract: | A metabonomic strategy based on LC-MS was employed to investigate the metabolic profile of urine samples from 20 athletes who had been tested positive for corticoids and anabolic steroids and 29 controls. In this aim, different sample preparations and chromatographic conditions were compared. The acquired LC-MS data of doped athletes and controls were subjected to analysis of variance (ANOVA) and principal component analysis (PCA). Using this approach, molecular signature of human urine was obtained showing that metabonomics could be a complementary tool to discriminate different urinary profiles and to track down metabolic changes in humans. |
| Keywords: | Metabolic profiling Metabonomics Metabolomics Doping products LC– MS Corticoids Anabolics |
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