半制备型柱与饼性能的比较

 通过比较具有相同柱几何体积的半制备柱和饼的性能 ,发现在同样的条件下 ,用液相方法分离生物大分子时 ,在分离效率和蛋白活性回收方面 ,饼和柱的差异不大 ;由于饼中的填料较柱中的装填更为紧密和均匀 ,当流动相流速增加时 ,饼不仅反向压力上升慢 ,而且质量和体积负载也比柱高。该结果表明使用饼的分离不仅具有可与高效液相 (HPLC)媲美的分离效率 ,而且还具有中、低压分离的高柱负载 ,这有利于蛋白保持高的生物活性 ,为饼的工业化应用奠定了基础。

Comparison of the Separation Performances between Semi-Preparative Chromatographic Column and Chromatographic Pie

By comparing the resolution and recoveries of proteins between semi-preparative chromatographic column and chromatographic pie (pie-like column) having the same geometric volume under the same chromatographic conditions, the result from the former was found to have the almost same as that form the latter. However, the back pressure of the chromatographic pie rose slower than that of the chromatographic column with the increase of flow rate. Furthermore, the mass and volume loads for the chromatographic pie were higher than those for the chromatographic column. The results indicated that the chromatographic pie had more advantages than the chromatographic column in their performances, and this lay a foundation of the applications of the chromatographic pie in industry.